5 in mice, at which time point erythropoiesis and myelopoiesis predominate in the presumptive red pulp, persisting until one week after birth 12, 13, while the structure of the white pulp surrounded by the marginal sinus gradually becomes organized with the proper positioning of T and B cell areas after birth 14 discreet cialis meds Total RNA was isolated from murine ventricles using TRIzol reagent Invitrogen 35, per the manufacturer instructions, and DNAse treated using the TURBO DNA free Kit Ambion